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Aflatoxin Redux: Does European Aflatoxin Regulation Hurt Groundnut Exporters from Africa? AgEcon
Xiong, Bo; Beghin, John C..
Replaced with revised version of paper 07/02/10.
Tipo: Conference Paper or Presentation Palavras-chave: Food safety; Standards; Aflatoxin; Maximum Residues Limit; Groundnut; Gravity equation; Africa; Market access; EU; Agricultural and Food Policy; Community/Rural/Urban Development; Crop Production/Industries; Food Consumption/Nutrition/Food Safety; Food Security and Poverty; International Development; International Relations/Trade; Q17; F13.
Ano: 2010 URL: http://purl.umn.edu/61314
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Aflatoxin removal of peanut meals with aqueous ethanol Scientia Agricola
Fonseca,H.; Regitano-d'Arce,M.A.B..
A preliminary approach to achieve compatible simultaneous extraction of aflatoxin and residual oil from pressed oil meals was conducted to determine the minimum amount of water to be added to ethanol versus necessary time to achieve complete removal of aflatoxin. Commercial anhydrous, 96, 93 and 90°GL ethanol were utilized in trials with Soxhlet extractors. Commercial anhydrous ethanol did not remove aflatoxin completely and the extraction efficiency in minutes was directly proportional to the amount of water present in the ethanol, as expected. Although 96°GL ethanol was efficient, alcoholic strengths between 96 and 99°GL need be evaluated to improve oil extraction, provided they do not decrease aflatoxin removal efficiency.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Peanut meal; Oil meals; Removal; Extraction; Aqueous ethanol; Ethanol.
Ano: 1993 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161993000100021
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Association of the severity of tongue lesions in broiler chickens with the quality of corn in feed PAB
Vaccari,Isabel Cristina Mores; Duarte,Vinicius; Rodrigues,Denise Russi; Santos,Fabiana Ramos dos; Minafra,Cibele Silva.
Abstract: The objective of this work was to determine the correlation of the physical attributes of corn and the mycotoxins present in its grains with the severity of tongue lesions in broiler breeder roosters. The occurrence of the aflatoxin, vomitoxin, zearalenone, T-2 toxin, and fumonisin mycotoxins was detected in the corn grains used in the feed formulation for breeder chickens. The percentages of moisture and broken grains were positively correlated (Pearson’s correlation) with the presence of aflatoxin. Milder lesions (grade 1) are positively correlated with aflatoxin and negatively correlated with fumonisin, and the most severe lesions (grade 3) are positively correlated with vomitoxin, fumonisin, and T-2 toxin.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Aviculture; Fumonisin; Mycotoxin; T-2 toxin; Vomitoxin..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017001000942
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Distribution of aflatoxins in contaminated corn fractions segregated by size BJM
Piedade,Fabiana S.; Fonseca,Homero; Glória,Eduardo M.; Calori-Domingues,Antonia; Piedade,Sônia M.S.; Barbin,Décio.
The efficiency of segregation, by particle size, in reducing the aflatoxin content of corn lots under the Brazilian conditions was studied. The aflatoxin content, in the corn fractions obtained after sieving thirty contaminated samples, with approximately 3.0 kg each, was determined. The samples were passed through 4.5 mm round-hole sieves and the fractions with particles ³4.5 mm (fraction A) and <4.5 mm (fraction B) and then analyzed for aflatoxins by TLC. The results showed that the distribution of the aflatoxins among the fractions A and B varied from sample to sample. However, the fraction B presented higher content (6 to 1,422 µg/kg) of aflatoxins (P<5%), than samples of fraction A (4 to 389 µg/kg). In spite of that the segregation of this...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Corn; Contamination distribution; Segregation; Fractions.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100002
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Effects of Yeast Glucomannan and Sodium Bentonite on the Toxicity of Aflatoxin in Broilers Rev. Bras. Ciênc. Avic.
Azizpour,A; Moghadam,N.
ABSTRACT The aim of this study was to evaluate the efficacy of a yeast glucomannan (YG) and sodium bentonite (SB) in reducing the toxicity of aflatoxin for broilers fed a diet naturally contaminated with aflatoxin. In total, 300 7-day-old Ross 308 broilers were randomly assigned to 10 dietary treatments, consisting of a diet with no aflatoxin (positive control), a diet naturally contaminated with 250 ppb aflatoxin (negative control), and eight aflatoxin-contaminated diets supplemented with different SB and YG and their combinations. Serum biochemical parameters and liver pathological changes were investigated when broilers were 42 days old. The birds fed the negative control diet presented lower uric acid, cholesterol, and triglyceride levels and higher in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Biochemical parameters; Broilers; Pathological changes; Yeast glucomannan.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015001000007
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Evaluation of yeast cell wall on the performance of broiles fed diets with or without mycotoxins Rev. Bras. Ciênc. Avic.
Santin,E; Paulillo,AC; Nakagui,LSO; Alessi,AC; Maiorka,A.
This experiment aimed at evaluating the effects of the interactions between aflatoxin (500 or 250 ppb) and ochratoxin (500 or 250 ppb), and the possible benefits of adding yeast cell wall to prevent the effects of these mycotoxins in broiler chickens. Relative organ weight gain and live performance were evaluated at 21 and 42 days of age. Results indicated that at the levels of mycotoxins included in the experimental diets, ochratoxin reduced feed intake and body weight gain, and aflatoxin only affect feed intake of 21-day-old birds. No interaction was observed between aflatoxin and ochratoxin at the levels used in experimental study. Yeast cell wall did not significantly reduced the deleterious effects of ochratoxins. No significant differences were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Ochratoxin; Yeast cell wall; Broilers; Performance.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000400004
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Identification of toxigenic Aspergillus species from diet dairy goat using a polyphasic approach Ciência Rural
Silva,Janaina Lara da; Aparecido,Christiane Ceriani; Hansen,Daiane; Pereira,Tacila Alves Muniz; Felicio,Joana D'arc; Gonçalez,Edlayne.
Some species of filamentous fungi that infest agricultural commodities are able to produce mycotoxins, contaminating feed and animal products. The aim of this research was to identify the mycoflora present in the feed and forage for dairy goat and to isolate and characterize the Aspergillus flavusand A. parasiticusstrains based on a morphological and molecular characterization and mycotoxigenic ability. The goat dairy diets were collected monthly from 11 goat milk farms, totaling 129 and 106 samples of concentrate and forage, respectively. For the isolation of the mycobiota the surface plating method was used. Aspergillus, Penicillium,and Fusariumwere the main fungi producing mycotoxins isolated. The morphological and molecular characterization and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Toxigenic fungi; Aflatoxin; Cyclopiazonic acid; Feed; Molecular characterization..
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000801466
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Immobilized Saccharomyces cerevisiae as a potential aflatoxin decontaminating agent in pistachio nuts BJM
Rahaie,S.; Emam-Djomeh,Z.; Razavi,S. H.; Mazaheri,M..
In this study, we investigated the binding ability of Saccharomayces cerevisiae to aflatoxin in pistachio nuts. The obtained results indicate that S. cerevisiae has an aflatoxin surface binding ability of 40% and 70% (with initial aflatoxin concentrations of 10 and 20 ppb) in the exponential phase. Acid treatments increase this ability to approximately 60% and 73% for the two concentrations of aflatoxin, respectively. Heat treatments also enhance surface binding to 55% and 75%, respectively. Binding appears to be a physical phenomenon that saturates within the first 2-3 hours of the process. The obtained results indicate that yeast immobilization for toxin reduction on aflatoxin-contaminated pistachios had no effect on qualitative characteristics, such as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Pistachio nut; Saccharomayces cerevisiae; Surface binding.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100014
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Inhibitory activity of compounds isolated from Polymnia sonchifolia on aflatoxin production by Aspergillus flavus BJM
Pak,Adriana; Gonçalez,Edlayne; Felicio,Joana D'arc; Pinto,Marina Mori; Rossi,Maria Helena; Simoni,Isabela Cristina; Lopes,Márcia Nasser.
Polymnia sonchifolia, commonly known as ";yacon";, was originally cultivated at Andes moutains in South America. Recently, the specie attracted worldwide attention because of its wide range of uses, for example in the control of diabetes melitus, besides the antifungal and pesticidal compounds were found in the leaves. This study describes the identification of two flavonoids: 3', 5, 7 trihydroxy-3, 4'-dimethoxyflavone (compound 1) and 3', 4', 5- trihydroxy-7-methoxy flavanone (compound 2) and two sesquiterpenes lactones: enhydrin (compound 3) and a mixture of enhydrin and uvedalin (compound 4) isolated from Polymnia sonchifolia leaves and their effects on the aflatoxin production by Aspergillus flavus. The identification of the compounds were achieved by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polymnia sonchifolia; Flavonoids; Melampolides; Aflatoxin; Aspergillus flavus.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200018
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Minimizing of Aflatoxin in Grain in the Tropics Thai Agricultural
Siriacha, P..
Palavras-chave: Aflatoxin.
Ano: 1999 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5033
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Molecular characterisation of Aspergillus flavus isolates from peanut fields in India using AFLP BJM
Singh,Diwakar; Radhakrishnan,T.; Kumar,Vinod; Bagwan,N.B.; Basu,M.S.; Dobaria,J.R.; Mishra,Gyan P.; Chanda,S.V..
Aflatoxin contamination of peanut, due to infection by Aspergillus flavus, is a major problem of rain-fed agriculture in India. In the present study, molecular characterisation of 187 Aspergillus flavus isolates, which were sampled from the peanut fields of Gujarat state in India, was performed using AFLP markers. On a pooled cluster analysis, the markers could successfully discriminate among the ‘A’, ‘B’ and ‘G’ group A. flavus isolates. PCoA analysis also showed equivalent results to the cluster analysis. Most of the isolates from one district could be clustered together, which indicated genetic similarity among the isolates. Further, a lot of genetic variability was observed within a district and within a group. The results of AMOVA test revealed that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; AMOVA; ELISA; Genetic diversity; Groundnut; PCA.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300673
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Mycotoxin contamination of diets for beef cattle finishing in feedlot R. Bras. Zootec.
Custódio,Letícia; Prados,Laura Franco; Yiannikouris,Alexandros; Holder,Vaughn; Pettigrew,James; Kuritza,Leandro; Resende,Flávio Dutra de; Siqueira,Gustavo Rezende.
ABSTRACT The objective of this survey was to identify what mycotoxins were present in ingredients used in diets offered to beef cattle in feedlots and their concentrations. The survey covered 30 Brazilian feedlots located in the five major beef-producing states. Samples of total mixed ration (TMR) and ingredients were collected and analyzed for mycotoxin using ultra-performance liquid chromatography coupled to tandem mass spectrometry. Mycotoxin concentrations were further interpreted according to known species-specific sensitivities and normalized according to the principles of toxic equivalent factors (risk equivalent quantities - REQ) expressed in µg kg−1 of aflatoxin B1-equivalent. Forty percent of the visited feedlots had apparent fungi in TMR....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Beef cattle; Feedlots; Fumonisin; Ingredients.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100724
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Nutritional changes in powdered red pepper upon in vitro infection of Aspergillus flavus BJM
Tripathi,Smita; Mishra,H.N..
Quantitative losses in various biochemical constituents like capsaicin, carotenes, ascorbic acid, polyphenols, mineral matter, sugars (soluble and insoluble), protein and fat were estimated after the successful growth of Aspergillus flavus for 30 days on powdered red pepper. The fungal biomass was measured by ergosterol content and Aflatoxin B1 by HPLC. Amongst the various nutritional constituents evaluated for nutritional losses and changes the highest nutritional loss was reported in total carotenoids (88.55%) followed by total sugars (85.5%). The protein content of the infected sample increased from 18.01% to 23%. The nutritional profile of chilli powder (Capsicum annum var. sannam L.) shows highest share of total soluble sugars (32.89%) and fiber...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus flavus; Chili powder; Nutritional changes; Aflatoxin.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100024
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Occurrence of mycootoxins in alfalfa (Medicago sativa L.), sorghum [Sorghum bicolor (L.) Moench], and grass (Cenchrus ciliaris L.) retailed in the state of Nuevo León, México Agrociencia
Huerta-Treviño,Alejandra; Dávila-Aviña,Jorge E.; Sánchez,Eduardo; Heredia,Norma; García,Santos.
Abstract Mycotoxins are secondary metabolites produced by specific filamentous fungi that are common contaminants of agricultural commodities. These compounds are toxic to humans and animals, and they are a health problem worldwide. Mycotoxin-containing feeds can cause serious diseases in farm animals and substantial economic losses. Therefore, the objective of this study was to determine the occurrence and levels of six mycotoxins in three types of forage at retail in the state of Nuevo León, México. The hypothesis was that all types of forages were contaminated with at least one of the six mycotoxins analyzed. One hundred and twenty samples of alfalfa (Medicago sativa L.), sorghum [Sorghum bicolor (L.) Moench], and grass (Cenchrus ciliaris L.) used for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycotoxins; Forages; Aflatoxin; Fumonisin; Ochratoxin.
Ano: 2016 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952016000700825
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Performance and egg quality of laying hens fed diets containing aflatoxin, fumonisin and adsorbent Rev. Bras. Ciênc. Avic.
Siloto,EV; Sartori,DRS; Oliveira,EFA; Sartori,JR; Fascina,VB; Berto,DA.
The effects of aflatoxin and fumonisin and their combination on egg production and quality, as well as the efficacy of a mycotoxin adsorbent in reducing or eliminating these effects in commercial layers. A number of 168 layers with initial age of 37 weeks were submitted to an experimental period of 56 days. A completely randomized experimental design in a 3x2+1 factorial arrangement was applied (3 treatments with mycotoxins: aflatoxin (AF), fumonisin (FU), or aflatoxin + fumonisin (AF+FU); 2 treatments with or without adsorbent; and a control group that was fed no mycotoxins, nor adsorbent), totaling 7 treatments with 6 replicated of 4 birds/cage. The dietary inclusion levels were 1ppm AF, 25ppm FU, and 2 kg adsorbent/ton feed. Birds fed AF presented the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adsorbent; Aflatoxin; Fumonisin; Layers; Performance.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2011000100004
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Production of aflatoxins by Aspergillus flavus and of fumonisins by Fusarium species isolated from Brazilian sorghum BJM
Silva,Josefa B. da; Dilkin,Paulo; Fonseca,Homero; Corrêa,Benedito.
Fifty-nine Aspergillus flavus and 35 Fusarium verticillioides strains, isolated from freshly harvested (10) and stored (130) Brazilian sorghum samples, were tested regarding their ability to produce aflatoxins (coconut milk agar) and fumonisins (rice culture), respectively. Aflatoxins B1 and B2 were detected by TLC, and fumonisins B1 and B2 were analyzed by HPLC. Thirty-eight (64.4%) A. flavus strains produced detectable levels of aflatoxins at concentrations ranging from 12.00 to 3282.50 µg/kg (AFB1 + AFB2), while thirty two (91%) F. verticillioides strains produced FB1 at concentrations ranging from 0.12 to 5.38 µg/g. Two F. proliferatum strains produced low fumonisin levels. The toxigenic potential of A. flavus (64.4%) and F. verticillioides (91.5%)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Toxigenicity; Aspergillus flavus; Fusarium verticillioides; Mycotoxins; Sorghum; Aflatoxin; Fumonisin.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200002
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Risk assessment of the occurrence of aflatoxin and fungi in peanuts and cashew nuts BJPS
Kujbida,Paula; Maia,Patrícia Penido; Araújo,Ariadne Naama de; Mendes,Leonardo Daniel; Oliveira,Mariana Lepri de; Silva-Rocha,Walicyranison Plinio; Brito,George Queiroz de; Chaves,Guilherme Maranhão; Martins,Isarita.
In the present study, the occurrence of fungi and aflatoxins (AFs) in peanut and cashew nut samples was investigated. Mycological analysis revealed the presence of fungi in 58.8% of samples, and assessment of AFs by chromatographic methods revealed that 52.9% were contaminated by AFs. AFB1 was the principal component in all AF-contaminated samples, with a mean level of 14.0, and 1.08 µg/kg in peanut and cashew nut, respectively. Eleven samples (32.4%) exceeded the total AF maximum level (4 μg/kg) and 8 samples (23.5%) exceeded the AFB1 (2 μg/kg) established by the European Commission. Our findings suggest that the incidence of AFs emphasizes the need for regular monitoring and a more stringent food safety system to control AFs at the lowest possible levels...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Peanut; Cashew nut; Fungi; Risk assessment.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100502
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Sampling plan for the analysis of aflatoxin in peanuts and corn: an update BJM
Fonseca,Homero.
The aim of this paper was to update the sampling plan for analysis of mycotoxins in grains, formerly published by the author. The proposed alterations were based on the acquired experience on its application and on FAO recommendations. This update restricts the scope of the former plan and establishes a sampling plan for analysis of aflatoxin in peanuts and corn, by means of modified formulas, the minimum number of sacks or points (when in bulk) from which incremental samples should be drawn to make a bulk sample. Fractional exponents (square roots) of the formulas proportionally decrease the number of sacks/points to be sampled as the lot size increases. Operating Characteristic (OC) curves developed for in-shell and shelled peanuts and corn as well as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sampling; Peanuts; Corn; Aflatoxin; Analysis.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200001
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The application of PCR in the detection of mycotoxigenic fungi in foods BJM
Konietzny,Ursula; Greiner,Ralf.
It is estimated that 25 to 50% of the crops harvested worldwide are contaminated with mycotoxins. Because of the toxic and carcinogenic potential of mycotoxins, there is an urgent need to develop detection methods that are rapid and highly specific. The highly advanced physico-chemical methods for the analysis of mycotoxins in use, have the disadvantage that highly sophisticated clean-up and/or derivatization procedures must be applied. An alternative could be the detection of the mycotoxigenic moulds themselves, especially as molecular techniques have been introduced recently as powerful tools for detecting and identifying fungi. PCR methods for the detection of aflatoxigenic Aspergilli, patulin-producing Penicillum and trichothecene- as well as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Fumonisin; Patulin; Polymerase chain reaction; Trichothecene.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400001
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The potential effects of Zataria multiflora Boiss essential oil on growth, aflatoxin production and transcription of aflatoxin biosynthesis pathway genes of toxigenic Aspergillus parasiticus BJM
Yahyaraeyat,R.; Khosravi,A.R.; Shahbazzadeh,D.; Khalaj,V..
This study aims at evaluating the effects of Zataria multiflora (Z. multiflora) essential oil (EO) on growth, aflatoxin production and transcription of aflatoxin biosynthesis pathway genes. Total RNAs of Aspergillus parasiticus (A.parasiticus) ATCC56775 grown in yeast extract sucrose (YES) broth medium treated with Z. multiflora EO were subjected to reverse transcription-polymerase chain reaction (RT-PCR). Specific primers of nor-1, ver-1, omt-A and aflR genes were used. In parallel mycelial dry weight of samples were measured and all the media were assayed by high-pressure liquid chromatography (HPLC) for aflatoxinB1 (AFB1), aflatoxinB2 (AFB2), aflatoxinG1 (AFG1), aflatoxinG2 (AFG2) and aflatoxin total (AFTotal) production. The results showed that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zataria multiflora; Reverse transcription-polymerase chain reaction; Aflatoxin; Aspergillus parasiticus.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200045
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